Hot start taq dna polymerase is a mixture of taq dna polymerase and an aptamerbased inhibitor. This lowtemperature polymerase activity is the basis for nonspecific amplification associated with mispriming events that occur during the initial phase of. Promega go taq master mixes prm7123 print share promega go taq master mixes. The following file naming structure is used to name the majority of these document files. Dna polymerase and taq dna polymerases from other vendors b promega gotaq g2 dna polymerase, c bioline mytaq dna polymerase, d neb onetaq dna polymerase, e takara taq dna polymerase, and f kapa biosystems taq pcr kit according to manufacturers recommendations. Cycling times for each polymerase are shown in purple, while ramping times on the proflex pcr system. Polymerase chain reaction, 122004 2 materials template dna genomic, plasmid, bacterial colony, etc. First isolated from the bacterial thermophile thermus aquaticus, the taq polymerase has become the staple enzyme in molecular biology. Since it was first isolated, taq dna polymerase has become the standard reagent for the pcr reaction. Certificate of analysis gotaq hot start polymerase.
Basic principles and chemistries leta steffen, phd applications scientist 20 promega corporation. Gotaq long pcr master mix a is a readytouse solution for long pcr targets. Add 95 ul water for every 5 ul primer to obtain 5 um solution. Efficiency comparison of seven different taq polymerases. Dreamtaq hot start green dna polymerase 200 u 500 u 2,500 u 4 x 2,500 u ep1711 ep1712.
Promega corporation 2800 woods hollow roadmadison, wi 537115399 u. The gene has been cloned and used to produce the enzyme in nonthermophilic host bacteria so both native taq, isolated from thermus aquaticus, and cloned taq, isolated from expression systems in other bacteria, are commercially. Use of taq polymerase as model system is a good way to introduce ip patent system, licensing issues, how patents are structured, and recent changes to patent system. Gotaq qpcr master mixa,b is a reagent system for quantitative pcr qpcr. Promega pcr product we will send a replacement or refund your account. Polymerase chain reaction pcr is a rapid procedure for in vitro enzymatic amplification of specific dna sequences using two oligonucleotide primers that hybridize to opposite strands and flank. Enhanced performance over standard taq dna polymerase.
New formulation of taq dna polymerase with enhanced performance over standard taq dna polymerase. The master mix contains everything needed to perform long pcroptimized buffer, dntps, mgcl 2 and an optimized hotstart enzyme blend for long pcr. Gotaqr pcr core system technical bulletin tb254 promega. The onetaq reaction buffers and high gc enhancer have been formulated for robust yields with minimal. The supplied 5x green and colorless flexi reaction buffers do not contain magnesium. The thermostable thermus aquaticusdna polymerase taq pol has been the key factor in transforming the initial pcr method into one with huge impact in molecular biology and biotechnolo.
Thermo scientific generuler 1 kb plus dna ladder was used. Combine the appropriate multiples of these components except template and dispense aliquots of the appropriate volume, minus the specific volume to be occupied by the template dna, to each reaction tube. Taq dna polymerase and taq pcr core kit en print bookmark share pdf 63kb english format file size language download get adobe reader contact qiagen. The enzyme consists of a single polypeptide with a molecular weight of 94 kda. Gotaq dna polymerase product information promega corporation.
All our enzymes are tested extensively for the absence of nicking and priming activities, exonucleases. Efficiency comparison of seven different taq polymerases used in hemogenetics j. Gotaq g2 dna polymerase reliably amplifies a wide range of pcr templates and. The 5x green gotaq reaction buffer contains two dyes blue and yellow that. This powerful polymerase mixture is designed to generate pcr products of high yield, high fidelity and high specificity from all types of dna 1. Taq is available with different formats to accommodate a variety of pcr applications. White paper platinum ii taq hotstart dna polymerase platinum ii taq hotstart dna polymerase for highthroughput pcr platinum ii taq hotstart dna polymerase is an innovative pcr reagent designed to address these challenges. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible.
High performance dna polymerases are purified according to multiple purification protocols minimizing the amount of e. It is supplied with 10x standard taq reaction buffer, which is detergentfree and designed to be compatible with existing assay systems. Production et purification rapide dune taq polymerase pour les. Gotaqr qpcr master mix technical manual tm318 promega. Visit our product page for additional information and protocols. Gotaq dna polymerase a,b is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. Gotaqr flexi dna polymerase product information sheet 9pim829.
Phusion dna polymerases also produce higher yields while using less enzyme than traditional proofreading polymerase reactions. Toll free in the usa 8003569526 telephone 6082744330 internet. A unique 10x reaction buffer for use with taq dna polymerase in reaction systems which require a detergent free. Comes with qiagen hotstartaq dna polymerase kit mix equal volumes of 100 mm stocks of invitrogen datp, dttp, dctp and dgtp primer stocks are at 100 um. Commercial producers have sinced cloned and expressed the enzyme in e. Pdf reverse transcriptase inhibits taq dna polymerase. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak.
Because taq dna polymerase is a nonproofreading polymerase, pcr products generated with taq dna polymerase will contain a singlenucleotide 3. Onetaq dna polymerase is an optimized blend of taq and deep vent dna polymerases for use with routine and difficult pcr experiments. The 5x green gotaq flexi buffer contains two dyes a blue dye and a yellow dye that separate during electrophoresis to. There are three main patents relating to the pcr process and taq dna polymerase. Purification of taq dna polymerase for 1 liter culture modified from the protocol presented in f.
Gotaq g2 dna polymerase product informationpdf 191 kb english. Taq dna polymerase is the industry standard for routine pcr. Promega go taq master mixes gotaq green master mix. Expand high fidelity pcr system is composed of a special enzyme mix containing thermostable taq dna polymerase and tgo dna polymerase, a thermostable dna polymerase with proofreading activity. The system contains a fluorescent the system contains a fluorescent dnabinding dye, the bryt green dye, that exhibits greater fluorescence enhancement upon binding to double. This high processivity results in shorter extension times, more robust amplification and the ability to amplify long templates up to 20 kb in a fraction of the time. Taq dna polymerase is a thermostable enzyme that synthesizes dna from singlestranded templates in the presence of dntps and a primer. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified dna from the pcr. The green master mix allows direct gel analysis after amplification.
Neb provides high quality recombinant taq at an exceptional value. Reverse transcriptase inhibits taq dna polymerase activity article pdf available in nucleic acids research 207. Primers resuspended in sterile water or te to a concentration of 100 mm buffer usually 10x, usually sold with taq polymerase or you can make your own. Antibodybased hot start taq ab taq addressed many of the concerns with prior approaches, and soon became a. Gotaq dna polymerase m300 protocolpdf 207 kb english. The 5x green gotaq reaction buffer contains two dyes blue and yellow that separate during electrophoresis to monitor migration progress. Like standard taq, it has both 5 to 3 polymerase and 5 to 3 exonuclease activity, but lacks 3 to 5 exonuclease activity. Gotaq green master mix product information promega corporation. The ptactaq vector, carrying the taq pol coding region was a kind gift of dr. Janica b a central forensic laboratory of the police, warsaw, poland b department of forensic medicine, medical university of bialystok, poland abstract. Gotaq dna polymerase is a proprietary formulation of taq polymerase that gives robust amplification equal to and, in some cases, superior to that of standard taq the 5x gotaq green and colorless reaction buffers supplied with gotaq dna polymerase contain mgcl 2 at a concentration of 7. Only the template and primers need to be added to amplify your target.
Pcr kit, b neb onetaq hot start dna polymerase, c promega gotaq g2 dna polymerase, d toyobo quick taq hs dyemix, e roche faststart taq dna polymerase, and f sigmaaldrich jumpstart taq dna polymerase. Gotaq green master mixa,b is a premixed readytouse solution containing bacterially derived taq dna polymerase, dntps, mgcl2 and reaction. Taq polymerase for robust pcr with and directtogel convenience. Currently, str typing is the most efficient and the fastest way for identification of. The enzyme is a taq dna polymerase supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification.
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